Unless your coursework was done at Ohio University, the answer is yes. Graduate College requires an actual transcript to be able to evaluate your application (in order to qualify as a transcript, the document has to bear the Registrar’s signature and/or the seal of the institution). If you are an OHIO alumnus, be sure to indicate that on your application and Graduate College staff will request your transcript from the Office of the Registrar.
If you have a copy of your official transcript in your possession, you can upload a digital copy of it to be used to evaluate your application. But if you do so, we still will need an official transcript to be sent directly from any other before the end of your first class).
Here are instructions from the Graduate College website:
You can submit an official transcript by mail or electronically, depending on the options provided by your school. Most institutions will have instructions online about how to request transcripts. Generally this information will be in the registrar's office or records office, but you may also find it under Alumni information.
Electronic transcript services:
Select Ohio University as the recipient, if that option is available. If asked to provide an e-mail address, use firstname.lastname@example.org.
Along with your name, please include your OHIO PID number on your order request.
Mailing paper transcripts:
Paper transcripts must arrive still sealed in the original envelope in which they were released by the issuing institution. The mailing address needs to include your full name and your Ohio PID number and/or your date of birth (month/date/year).
Your Name, Pxxxxxxxxx
1 Ohio University
Grosvenor Hall 102
Athens OH 45701