Ohio University Genomics Facility

BRIEF DESCRIPTION OF FACILITY:

The Ohio University Genomics Facility is committed to enhancing the research, education, and prestige of the Ohio University Community.

CAPABILITIES OF FACILITY:

The OUGF houses several pieces of shared equipment:

• AB 3130xl Genetic Analyzer 16 capillary array
• Nanodrop 1000
• Agilent 2100 Bioanalyzer
• Agilent 3100 Off Gel Fractionator
• Sage Science Pippin Prep
• Stratagene MX3000P Real Time PCR machine
• Illumina MiSeq
• Ion Torrent PGM

Each of the above listed pieces of equipment are available for use by any member of OU as well as researchers from other universities, government, and industry.

The services offered by the OUGF include:

• DNA sequencing, fragment and microsatellite analysis, AFLP, SNP screening
• RNA/DNA quantification, quality control, size distribution, RIN, sRNA analysis
• Protein fraction sizing and quanitification, QA/QC purified protein/antibodies
• Low cell consumption two-color flow cytometry analysis
• Isoelectric focusing prior to Mass Spec, DIGE analysis, or SDS-PAGE
• Automated nucleic acid separation and size collection (90 bp to 1.5 kbp)
• Relative and Absolute qPCR quantification (SYBR, TaqMan probe)
• End-point PCR genotyping, fragment melt analysis, copy number determination
• Next-Generation Sequencing including:
  • Total RNA, small RNA, exosome and rRNA-depleted RNAseq
  • SHAPE-seq, 5’-end mapping
  • de novo genome sequencing and targeted DNA sequencing
  • ChIP-seq, Methyl-seq
• Bioinformatic analysis of NGS data
• Custom experimental design, optimization, and consulting

In addition to what is listed here, the OUGF also offers tours of the facility for anyone interested in seeing the space and learning more about our capabilities.

TRAINING/TECHNICAL HELP AVAILABLE OR REQUIRED TO USE FACILITY EQUIPMENT?

Yes.

FEES FOR USE OF EQUIPMENT?

Some equipment is free for use; others do have a fee. Please contact for Nigel Daniels for exact pricing.

Genomics Website: https://www.ohio.edu/cas/genomics

Revised 04/25/23