Molecular and Cellular Biology Laboratory

MCB7300: Molecular & Cellular Biology Laboratory

The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.


		MCB7300: Molecular & Cellular Biology Laboratory The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory. Syllabus 2015 Revised Syllabus 2015 Day 1 Introduction to the Wyatt lab module (read web links below): Bitesize web link Life technologies web link Flowchart-Wyatt 2014 Lab 1 Genomic DNA Extraction Lab 2 Ligation Protocol Lab 2 TA Cloning Lab 2 pCR TOPO map Lab 3 Preparation of Plasmid DNA Lab 4 Restriction Enzyme Digestions and Mapping RNA and qPCR lab 2015- PowerPoint RNA isolation protocol cDNA synthesis and qPCR setup protocol GoScript RT system RNeasy Mini Handbook SYBR Green QPCR Southern blot lab 2015-PowerPoint Southern blot lab 2015: Backgound, Overview and Detailed Protocol Southern blot lab: DIG labeling manual-starter kit I (28 pages) Southern blot lab: DIG labeling manual (starter kit II) Bioinformatics lab 1 Assignment Bioinformatics lab 2 Assignment Bioinformatics lab slides Information below comes from labs in prior years: Syllabus 2014 Southern blot lab 2014-PowerPoint Southern blot lab 2014: Backgound, Overview and Detailed Protocol Southern blot lab: DIG labeling manual (starter kit II) Southern Blotting Lab Results-March 2014-blots Southern Blotting Lab Results-March 2014-colonies #1 Southern Blotting Lab Results-March 2014-colonies #2 Syllabus 2013 Flowchart-Briju 2013 Lab 1 genomic DNA Extraction Lab 2 Ligation Protocol Lab 3 Minipreps Lab 4 Restriction Analysis Bioinformatics Lecture 1 Bioinformatics Lecture 2 Bioinformatics Assignment 1 Bioinformatics Assignment 2 Southern blot lab 2013-PowerPoint Southern blot lab: Backgound, Overview and Detailed Protocol Southern blot lab: DIG labeling manual-starter kit I (28 pages) Southern Blotting Lab Results-March 2013 Western Blotting Lab 2013-PDF/Power Point Western Blotting Lab Result-PowerPoint Image-March 2013 Laser Microdissection lab 2013-PowerPoint 1 Laser Microdissection lab 2013-PowerPoint 2 Laser Microdissection lab 2013-Paper Laser Microdissection lab 2013-Images Fluorescence microscopy lab 2013 Syllabus 2011 Bioinformatics 1-Chen 2011-powerpoint Bioinformatics 2-Chen 2011-powerpoint Bioinformatics 1 Assignment-Chen 2011 Bioinformatics 2 Assignment-Chen 2011 Flowchart-Wyatt 2010 Lab 1 genomic DNA Lab 2 ligation etc. Lab 2 protocol Lab 3 minipreps, RE, gel Carbohydrate composition analysis using GC-MS Western blot lab 2011-PowerPoint Western blot lab 2011-Results Southern blot lab 2011-PowerPoint Southern blot lab: Backgound, Overview and Detailed Protocol Southern blot lab: DIG labeling manual (28 pages) Fluorescence Microscopy Lab-Berryman 2011 Fluorescence Microscopy Lab-Berryman 2011-PowerPoint Microscopy Lab-Duerr 2011 (includes a pre-lab quiz) Syllabus 2010 Flowchart-Wyatt 2010 Lab 1 genomic DNA Lab 2 ligation etc. Lab 2 protocol Lab 3 minipreps, RE, gel Western blot lab 2010-powerpoint Southern blot lab 2010-powerpoint Southern blot lab: Backgound, Overview and Detailed Protocol Southern blot lab: DIG labeling manual (28 pages) Syllabus 2009 Southern blot labs: Powerpoint-2009 Southern blot labs: Flowchart Southnern blot labs: Detailed Protocol Southnern blot labs: Primers and Probe Sequence Syllabus 200 7 Flowchart of Labs 1-7 Lab 1 genomic DNA Lab 2 ligation Lab 2 Protocol Labs 3and 4 southern Lab 5 minipreps Lab 6 and 7 DNA sequencing Showalter-PCR Lab 2007 Promega’s GammaSTR® Fluorescent STR System Showalter Powerpoint: DNA Fingerprinting 2007 Showalter-PCR Lab Results 2007 (D1S80 & ACE) Showalter-PCR Lab Results 2007 (STR chromatograms) Showalter-PCR Lab Resuts 2007 (STR table) PCR Lab Results 2006 Showalter-RNA isolation & RT-PCR Lab Handout 2006 RT-PCR Lab Results 2006 QPCR Powerpoint-2006 Faik Bioinformatics PowerPoint 1-2006 Faik Bioinformatics PowerPoint 2-2006 Faik Bioinformatics Assignment 1-2006 Faik Bioinformatics Assignment 2-2006 Berryman Fluorescence Microscopy Lab 2006 SEM Pictures-Miller Lab 2006 TEM Picture-Miller Lab 2006 Western/Dot Blotting Lab 2006 Western Blotting Questions 2006
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