MCB7300: Molecular & Cellular Biology Laboratory
The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.
Syllabus 2015
Revised Syllabus 2015
Day 1 Introduction to the Wyatt lab module (read web links below):
Bitesize web link
Life technologies web link
Flowchart-Wyatt 2014
Lab 1 Genomic DNA Extraction
Lab 2 Ligation Protocol
Lab 2 TA Cloning
Lab 2 pCR TOPO map
Lab 3 Preparation of Plasmid DNA
Lab 4 Restriction Enzyme Digestions and Mapping
RNA and qPCR lab 2015- PowerPoint
RNA isolation protocol
cDNA synthesis and qPCR setup protocol
GoScript RT system
RNeasy Mini Handbook
SYBR Green QPCR
Southern blot lab 2015-PowerPoint
Southern blot lab 2015: Backgound, Overview and Detailed Protocol
Southern blot lab: DIG labeling manual-starter kit I (28 pages)
Southern blot lab: DIG labeling manual (starter kit II)
Bioinformatics lab 1 Assignment
Bioinformatics lab 2 Assignment
Bioinformatics lab slides
Information below comes from labs in prior years:
Syllabus 2014
Southern blot lab 2014-PowerPoint
Southern blot lab 2014: Backgound, Overview and Detailed Protocol
Southern Blotting Lab Results-March 2014-blots
Southern Blotting Lab Results-March 2014-colonies #1
Southern Blotting Lab Results-March 2014-colonies #2
Syllabus 2013
Flowchart-Briju 2013
Lab 1 genomic DNA Extraction
Lab 3 Minipreps
Lab 4 Restriction Analysis
Bioinformatics Lecture 1
Bioinformatics Lecture 2
Bioinformatics Assignment 1
Bioinformatics Assignment 2
Southern blot lab 2013-PowerPoint
Southern blot lab: Backgound, Overview and Detailed Protocol
Southern Blotting Lab Results-March 2013
Western Blotting Lab 2013-PDF/Power Point
Western Blotting Lab Result-PowerPoint Image-March 2013
Laser Microdissection lab 2013-PowerPoint 1
Laser Microdissection lab 2013-PowerPoint 2
Laser Microdissection lab 2013-Paper
Laser Microdissection lab 2013-Images
Fluorescence microscopy lab 2013
Syllabus 2011
Bioinformatics 1-Chen 2011-powerpoint
Bioinformatics 2-Chen 2011-powerpoint
Bioinformatics 1 Assignment-Chen 2011
Bioinformatics 2 Assignment-Chen 2011
Flowchart-Wyatt 2010
Lab 1 genomic DNA
Lab 2 ligation etc.
Lab 2 protocol
Lab 3 minipreps, RE, gel
Carbohydrate composition analysis using GC-MS
Western blot lab 2011-PowerPoint
Western blot lab 2011-Results
Southern blot lab 2011-PowerPoint
Southern blot lab: DIG labeling manual (28 pages)
Fluorescence Microscopy Lab-Berryman 2011
Fluorescence Microscopy Lab-Berryman 2011-PowerPoint
Microscopy Lab-Duerr 2011 (includes a pre-lab quiz)
Syllabus 2010
Western blot lab 2010-powerpoint
Southern blot lab 2010-powerpoint
Syllabus 2009
Southern blot labs: Powerpoint-2009
Southern blot labs: Flowchart
Southnern blot labs: Detailed Protocol
Southnern blot labs: Primers and Probe Sequence
Syllabus 2007
Flowchart of Labs 1-7
Lab 2 ligation
Lab 2 Protocol
Labs 3and 4 southern
Lab 5 minipreps
Lab 6 and 7 DNA sequencing
Showalter-PCR Lab 2007
Promega’s GammaSTR® Fluorescent STR System
Showalter Powerpoint: DNA Fingerprinting 2007
Showalter-PCR Lab Results 2007 (D1S80 & ACE)
Showalter-PCR Lab Results 2007 (STR chromatograms)
Showalter-PCR Lab Resuts 2007 (STR table)
PCR Lab Results 2006
Showalter-RNA isolation & RT-PCR Lab Handout 2006
RT-PCR Lab Results 2006
QPCR Powerpoint-2006
Faik Bioinformatics PowerPoint 1-2006
Faik Bioinformatics PowerPoint 2-2006
Faik Bioinformatics Assignment 1-2006
Faik Bioinformatics Assignment 2-2006
Berryman Fluorescence Microscopy Lab 2006
SEM Pictures-Miller Lab 2006
TEM Picture-Miller Lab 2006
Western/Dot Blotting Lab 2006
Western Blotting Questions 2006
Environmental and Plant Biology | OU Front Door