MCB7300: Molecular & Cellular Biology Laboratory

The purpose of this course is to expose students to a variety of laboratory techniques used in the broad field of molecular and cellular biology by allowing the student to carry out these techniques in the laboratory.

Syllabus 2015

Revised Syllabus 2015

Day 1 Introduction to the Wyatt lab module (read web links below):

Bitesize  web link

Life technologies web link

Flowchart-Wyatt 2014

Lab 1 Genomic DNA Extraction

Lab 2 Ligation Protocol

Lab 2 TA Cloning

Lab 2 pCR TOPO map

Lab 3 Preparation of Plasmid DNA

Lab 4 Restriction Enzyme Digestions and Mapping


RNA and qPCR lab 2015- PowerPoint

RNA isolation protocol

cDNA synthesis and qPCR setup protocol

GoScript RT system

RNeasy Mini Handbook

SYBR Green QPCR


Southern blot lab 2015-PowerPoint

Southern blot lab 2015: Backgound, Overview and Detailed Protocol

Southern blot lab: DIG labeling manual-starter kit I (28 pages)

Southern blot lab: DIG labeling manual (starter kit II)


Bioinformatics lab 1 Assignment

Bioinformatics lab 2 Assignment

Bioinformatics lab slides



Information below comes from labs in prior years:

Syllabus 2014

Southern blot lab 2014-PowerPoint

Southern blot lab 2014: Backgound, Overview and Detailed Protocol

Southern blot lab: DIG labeling manual (starter kit II)

Southern Blotting Lab Results-March 2014-blots

Southern Blotting Lab Results-March 2014-colonies #1

Southern Blotting Lab Results-March 2014-colonies #2


Syllabus 2013

Flowchart-Briju 2013

Lab 1 genomic DNA Extraction

Lab 2 Ligation Protocol

Lab 3 Minipreps

Lab 4 Restriction Analysis

Bioinformatics Lecture 1

Bioinformatics Lecture 2

Bioinformatics Assignment 1

Bioinformatics Assignment 2

Southern blot lab 2013-PowerPoint

Southern blot lab: Backgound, Overview and Detailed Protocol

Southern blot lab: DIG labeling manual-starter kit I (28 pages)

Southern Blotting Lab Results-March 2013

Western Blotting Lab 2013-PDF/Power Point

Western Blotting Lab Result-PowerPoint Image-March 2013

Laser Microdissection lab 2013-PowerPoint 1

Laser Microdissection lab 2013-PowerPoint 2

Laser Microdissection lab 2013-Paper

Laser Microdissection lab 2013-Images

Fluorescence microscopy lab 2013


Syllabus 2011

Bioinformatics 1-Chen 2011-powerpoint

Bioinformatics 2-Chen 2011-powerpoint

Bioinformatics 1 Assignment-Chen 2011

Bioinformatics 2 Assignment-Chen 2011

Flowchart-Wyatt 2010

Lab 1 genomic DNA

Lab 2 ligation etc.

Lab 2 protocol

Lab 3 minipreps, RE, gel

Carbohydrate composition analysis using GC-MS

Western blot lab 2011-PowerPoint

Western blot lab 2011-Results

Southern blot lab 2011-PowerPoint

Southern blot lab: Backgound, Overview and Detailed Protocol

Southern blot lab: DIG labeling manual (28 pages)

Fluorescence Microscopy Lab-Berryman 2011

Fluorescence Microscopy Lab-Berryman 2011-PowerPoint

Microscopy Lab-Duerr 2011 (includes a pre-lab quiz)


Syllabus 2010

Flowchart-Wyatt 2010

Lab 1 genomic DNA

Lab 2 ligation etc.

Lab 2 protocol

Lab 3 minipreps, RE, gel

Western blot lab 2010-powerpoint

Southern blot lab 2010-powerpoint

Southern blot lab: Backgound, Overview and Detailed Protocol

Southern blot lab: DIG labeling manual (28 pages)


Syllabus 2009

Southern blot labs: Powerpoint-2009

Southern blot labs: Flowchart

Southnern blot labs: Detailed Protocol

Southnern blot labs: Primers and Probe Sequence


Syllabus 2007

Flowchart of Labs 1-7

Lab 1 genomic DNA

Lab 2 ligation

Lab 2 Protocol

Labs 3and 4 southern

Lab 5 minipreps

Lab 6 and 7 DNA sequencing

Showalter-PCR Lab 2007

Promega’s GammaSTR® Fluorescent STR System

Showalter Powerpoint: DNA Fingerprinting 2007

Showalter-PCR Lab Results 2007 (D1S80 & ACE)

Showalter-PCR Lab Results 2007 (STR chromatograms)

Showalter-PCR Lab Resuts 2007 (STR table)


PCR Lab Results 2006

Showalter-RNA isolation & RT-PCR Lab Handout 2006

RT-PCR Lab Results 2006

QPCR Powerpoint-2006

Faik Bioinformatics PowerPoint 1-2006

Faik Bioinformatics PowerPoint 2-2006

Faik Bioinformatics Assignment 1-2006

Faik Bioinformatics Assignment 2-2006

Berryman Fluorescence Microscopy Lab 2006

SEM Pictures-Miller Lab 2006

TEM Picture-Miller Lab 2006

Western/Dot Blotting Lab 2006

Western Blotting Questions 2006

 


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