Biomedical Core Facilities
In accordance with the directive from the “stay at home” order issued by the Governor of Ohio, the Heritage College Histology and Mouse Metabolic Phenotyping Core Facilities will be closed until further notice. We may consider exceptions, but you will need to get permission in advance from the Heritage College Office of Research and Grants and the director of the core facility. Please submit requests through the director first and plan accordingly. As you know, we urge you to not start any new experiments until the order is lifted. Stay safe!
Histology Core Director: Dr. Ramiro Malgor email@example.com
Mouse Metabolic Phenotyping Core Director: Dr. Chunmin Lo firstname.lastname@example.org
The Microscopy Core has reopened. View guidance documents on the Microscopy Core page or contact the director, Dr. Mark Berryman email@example.com
The Heritage College Microscopy Facility provides use of a Nikon A1R confocal microscope with a resonant scanner, spectral detection and live imaging capabilities. This powerful system is fully automated and captures high-quality confocal images of cells and molecular events at high speeds with enhanced sensitivity. It can accommodate the needs of a broad range of users.
The Heritage College Histology Facility houses specialized equipment to process, embed, stain and section tissue samples for slides. In addition, laser capture microdissection and digital scanning services are available. Equipment reservations and technical training of users is required.
The faculty and staff of the Histology Core Facility are highly skilled and are able to offer the following services:
Paraffin Block Preparation
Hematoxylin & Eosin Staining
Other Histochemical Staining (i.e., PAS)
Cryostat Sections Training
Mouse Metabolic Phenotyping Core*
The Heritage College Mouse Metabolic Phenotyping Core Facility houses the Comprehensive Lab Animal Monitoring System (CLAMS) equipment that provides useful non-destructive methods for the determination of physiological roles in energy homeostasis and animal activity in live mice with different genotypes on a bench-top platform. These powerful systems are fully automated and monitor food and water intake, energy expenditure and animal activity (locomotor and voluntary activity) in mice before and after drug treatments in a controlled temperature and lighting environment.
Whole-body Composition Analyzer*
The Brubaker Minispec Mice Analyzer LF50 includes a nuclear magnetic resonance spectroscopy (NMR) probe, daily calibration and mouse restrainers. This system provides a precise method for measurement of lean tissue and fat and fluid in mice and carcass.
Ohio University's BD Special Order Research Product FACSAria II, a state-of-the-art flow cytometer/cell sorter, is equipped with two lasers and is capable of four-way cell sorting based on size, granularity and/or fluorescent signal. Up to eight colors can be analyzed, including but not limited to: APC, APC-Cy7, Alexa Fluor 488, Alexa Fluor-700, FITC, GFP, PE, PE-Cy7, PE-Texas Red, PerCP and PerCP-Cy5.5.
The flow cytometer is housed in the Academic & Research Center. Its purchase was made possible by the National Science Foundation under Grant No. 1039869 to the Russ College of Engineering and Technology, the College of Osteopathic Medicine and the College of Arts and Sciences.
Michelle Pate ( firstname.lastname@example.org )
597-1224 (9 a.m. to 12:30 p.m.)
The Ohio University MicroCT Scanner (OUµCT) allows non-destructive 3D X-ray imaging and digitization of objects smaller than about 10 cm diameter. Live animals (e.g., mice and rats) can be imaged and even instrumented, and the OUµCT provides cardiac and respiratory gating that reduces motion artifact. The scanner has a unique power generator that allows faster and cleaner imaging, which improves data quality while also reducing x-ray dose and cost. Resolution ranges from 25-100 microns. Specimen densities range from anatomical soft tissues to industrial applications.
*Equipment purchased with financial support provided by a grant from the Osteopathic Heritage Foundation's Vision 2020: Leading the Transformation of Primary Care in Ohio award.