Biomedical Core Facilities
In accordance with the directive from the “stay at home” order issued by the Governor of Ohio, the HCOM Microscopy and Histology Core Facilities will be closed until further notice. We may consider exceptions, but you will need to get permission in advance from the HCOM Research Office (ORG: Office of Research and Grants) https://www.ohio.edu/medicine/research/org and the director of the core facility. Please submit requests through the director first and plan accordingly. As you know, we urge you to not start any new experiments until the order is lifted. Stay safe!
Microscopy Core Director: Dr. Mark Berryman email@example.com
Histology Core Director: Dr. Ramiro Malgor firstname.lastname@example.org
The Heritage College Microscopy Facility provides use of a Nikon A1R confocal microscope with a resonant scanner, spectral detection, and live imaging capabilities. This powerful system is fully automated and captures high-quality confocal images of cells and molecular events at high speeds with enhanced sensitivity. It can accommodate the needs of a broad range of users.
Please review the following workplan for procedures in place during the COVID-19 pandemic and Ohio University's reduction of workforce density directive.
The Heritage College Histology Facility provides use of the following equipment:
Leica ASP300 Tissue Processor
Leica EG1160 Embedding System
Leica 1150H-EG1150C Embedding System
Leica RM2135 High Profile Microtome
Leica RM2235 Low Profile Microtome
Leica Automatic Stainer
Leica CM1950 Cryostat
The automatic tissue processor can process large number of tissue samples simultaneously, from buffered formalin fixed samples to paraffin. Both embedding systems are for paraffin blocks preparation. The high and low profile microtomes are for preparation of tissue section on slides. The cryostat cuts frozen tissues section.
In addition, the faculty and staff of the Histology Facility are highly skilled are able to offer the following services:
Parraffin Block Preparation
Hematoxylin Eosin Stain
Other Histochemical Staining (i.e. PAS, Trichrome)
Cryostat Sections Training
Director: Dr. Ramiro Malgor ( email@example.com )
Due to the high demand for equipment, reservations are required. In addition, technical training and registration of users is required.
Ohio University's BD Special Order Research Product FACSAria II, a state-of-the-art flow cytometer/cell sorter, is equipped with two lasers and is capable of four-way cell sorting based on size, granularity, and/or fluorescent signal. Up to eight colors can be analyzed, including but not limited to: APC, APC-Cy7, Alexa Fluor 488, Alexa Fluor-700, FITC, GFP, PE, PE-Cy7, PE-Texas Red, PerCP, and PerCP-Cy5.5.
The flow cytometer is housed in the Academic & Research Center. Its purchase was made possible by the National Science Foundation under Grant No. 1039869 to the Russ College of Engineering and Technology, the College of Osteopathic Medicine, and the College of Arts and Sciences.
Michelle Pate ( firstname.lastname@example.org )
597-1224 (9am to 12:30 pm)
The Ohio University MicroCT Scanner (OUµCT) allows non-destructive 3D x-ray imaging and digitization of objects smaller than about 10 cm diameter. Live animals (e.g., mice and rats) can be imaged and even instrumented, and the OUµCT provides cardiac and respiratory gating that reduces motion artifact. The scanner has a unique power generator that allows faster and cleaner imaging, which improves data quality while also reducing x-ray dose and cost. Resolution ranges from 25-100 microns. Specimen densities range from anatomical soft tissues to industrial applications.
*Equipment purchased with financial support provided by a grant from the Osteopathic Heritage Foundation's Vision 2020: Leading the Transformation of Primary Care in Ohio award.