Tomohiko Sugiyama
Recent News
Education
Ph.D., Osaka University, Osaka, Japan
Courses Taught
- BIOS3200 Fundamentals of Animal Cell Biology
- BIOS 2250 Genetics and Human Society
- BIOS 4635 Biological Chemistry lab
- BIOS 4630 Biological Chemistry lecture
Research Interests
- Lab: Life Science Building 240
We are studying molecular mechanisms of DNA repair and mutagenesis. By using biochemical methods and next-generations sequencing technology, we are analyzing the details of mutational processes that cause various types of cancer.
For example, UV radiation is well-known cause of skin cancer. We are working to reproducethe entire process of the UV-induced mutagenesis in vitro by using model DNA substrates and purified enzymes, hoping to reproduce typical mutation patterns (mutational signature) of skin cancer.
Many cancers have unique patterns of mutations that should be related to their etiologies. We will analyze more (potential) carcinogens to identify their mutation profiles and to understand biochemical mechanisms of the mutational processes. These studies will contribute for better understanding and for prevention of cancers.
Representative Publications
Ohio University students are bold, along with Dr. Sugiyama.
Billon, P., Li, J., Lambert, J.P., Chen, Y., Tremblay, V., Brunzelle, J.S., Gingras, A.C., Verreault, A., Sugiyama, T., Couture, J.F., et al. (2017). Acetylation of PCNA Sliding Surface by Eco1 Promotes Genome Stability through Homologous Recombination. Mol Cell 65, 78-90. doi: 10.1016/j.molcel.2016.10.033
Khade, N., and Sugiyama, T., (2016) Yeast recombination mediator Rad52 can load human Rad51 onto the single-strand DNA that is complexed with yeast RPA. PLOS ONE. DOI:10.1371/journal.pone.0158436 June 30
Li, J., Holzschu, D., Sugiyama, T., (2013) PCNA is efficiently loaded on the DNA recombination intermediate to modulate Pol δ, η, ζ activities. Proc. of the Natl. Acad. Sci. USA 110, 7672-7
Sugiyama, T*., Kantake, T., Wu Y., and Kowalczykowski, S. C. (2006) Rad52-mediated DNA annealing after Rad51-mediated DNA strand exchange promotes second ssDNA capture. EMBO J. 25, 5539–5548
Funding
NIH (2015-2019) R15 "NGS-based analysis of mutation signatures during the translesion synthesis". PI